FAQ
Straight answers for operators.
The questions cultivators actually ask before they bring tissue culture in-house.
How long does it take to build an in-house tissue culture lab?
About six months. The engagement covers facility assessment, clean-room design, equipment sourcing, SOP development, media formulation, hands-on training, and commissioning. The goal is independent operation. Construction schedules, cultivar response and screening results can affect when each milestone is reached.
What does building a tissue culture lab involve?
Facility assessment, clean-room design and layout, equipment sourcing, construction oversight with your contractors, customized SOPs, media formulation, hands-on staff training in sterile technique and micropropagation, and commissioning. You end with a working lab and a team that runs it.
What is HLVd, and can it be removed?
HLVd (hop latent viroid, also written HpLVd) is an infectious RNA pathogen that silently reduces cannabis potency, yield, and vigor. Meristem culture can be used to remediate HLVd by regenerating plants from very small shoot tips. Regenerated plants need qPCR screening and review before a line returns to production.
Do you offer HLVd remediation as a service?
Yes. We help your team establish a meristem-culture workflow for HLVd remediation in your facility, with qPCR screening of regenerated plants before a line returns to production. HLVd is also written HpLVd. We scope this as a standalone project or as part of a lab build or rehabilitation.
Will my own team actually be able to run the lab?
Yes. That is the entire model. We train your team hands-on and document every protocol so they run clean-stock production without ongoing dependence on a consultant. The engagement ends with your team self-sufficient.
What happens after you finish the build?
Your team owns and runs the lab. Optional ongoing support is available as a monthly retainer covering strategy calls, periodic on-site visits, protocol refinements, HLVd cleanup, and cold storage. It is optional, not required. The scope, initial term and renewal conditions are set out in your consulting agreement.
Who do you work with?
Licensed commercial cannabis cultivators and multi-state operators, in the United States and internationally, who want to bring tissue culture and clean-stock production in-house rather than outsourcing clone production. Recent clients include District Cannabis, Story Cannabis, Eastern Sierra Group, and Nimbus Farms.
How much does Clonedock cost?
Standalone Clonedock subscriptions are $800 a month, and the first 10 labs get a founding rate of $450 a month that is fixed for an initial two year term, after which no single renewal can raise it by more than 25%. Same product either way, with setup and onboarding included. Claiming a seat takes no card and costs nothing: we review each lab by hand, and standalone software billing is set up during onboarding and starts when your lab goes live. You do not need a cannabis license. Clonedock is included for the duration of a full Phase 1 lab build and implementation package. Continued access after Phase 1, including during Phase 2, requires a paid software subscription.
What results have you produced?
A lab built in six months for District Cannabis, a Story Cannabis program operational for nearly a year, and 500+ cultivars preserved across the country through tissue culture and cold storage.
Why bring tissue culture in-house instead of buying clones?
Outsourced clones are inconsistent and can reintroduce pathogens like HLVd. An in-house clean-stock program gives you control over genetic quality, consistency, and supply, and preserves your elite cultivars in cold storage so losing a room does not mean losing the genetics.
What drives the cost of an in-house tissue culture lab?
The cost of an in-house tissue culture lab is driven mostly by scale, equipment choices, and the state of the space you start with. A larger cultivar library and higher production volume need more hood time, more growth-chamber capacity, and more media throughput, which is where the money goes. A room that already has clean power, water, and environmental control costs less to convert than one built from scratch. We scope the real number to your facility on the consultation rather than quoting a generic figure.
What equipment does a tissue culture lab need?
A tissue culture lab needs a laminar flow hood for sterile work, an autoclave to sterilize media and tools, a growth room or chamber with controlled light and temperature, a media-prep area, and shelving and consumables. The hood does the heavy lifting on contamination control, and growth-chamber capacity is usually what caps how much you can produce. We source the right equipment for your scale so you are not over-buying or bottlenecked on day one.
How does meristem culture remove HLVd?
Meristem culture uses a very small shoot tip to regenerate a plant in tissue culture. It is used in HLVd remediation because it can produce lines that test negative, but results vary by cultivar and protocol. We use qPCR screening to evaluate regenerated plants before a line returns to production.
What is genetic cold storage and why does a lab need it?
Genetic cold storage is long-term preservation of clean plant tissue at low temperature so a cultivar can be regenerated years later. A lab needs it because it is insurance: if you lose your mother plants to a contamination event or a bad season, cold-storage tissue lets you bring those genetics back. It is how we have preserved 500+ cultivars across the country, so an elite cultivar is never one accident away from being gone for good.
What is the difference between a tissue culture lab and a mother room?
A tissue culture lab produces clean genetics in a sterile setting, while a mother room keeps stock plants for taking traditional cuttings. A mother room can quietly hold and spread HLVd across every clone you pull from it. A tissue culture lab starts clean, screens for pathogens, and regenerates Gen-0 stock that outperforms cuttings from older or infected mothers. The lab is upstream: it feeds clean plants into the room, instead of the room slowly degrading your library.
What is media, and why does it matter in tissue culture?
Media is the sterile nutrient gel that cannabis tissue grows on in the lab, and getting the recipe right is what makes propagation clean and consistent. Each stage, from initiation to multiplication to rooting, wants a different formulation, and small changes in the recipe show up as differences in vigor and contamination. We formulate media to your cultivars and train your team to prep and track it, because a lab that dials in its media is a lab that produces predictable clean stock.
Still have a question?
Ask it on a consultation. We will give you a straight answer for your facility.